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Genomics Core Library Preparation Submission
| Library prep kit | Submit | Submission workflow | Quantity range (ng) | Volume required per prep (uL)* | Sample batch size** | Notes |
|---|---|---|---|---|---|---|
| Illumina Stranded mRNA | Total RNA | Illumina Stranded mRNA | 25-1000 | 25 | Any number | This kit supports various input mass; normalise where possible. |
| NEBNext Single cell / low input RNA Library Prep Kit for Illumina | Total RNA/cells | NEB low input RNA | 2pg - 200ng, or cell(s) | 8 | 24 | Please discuss your input requirements prior to submission. |
| Illumina DNA PCR-Free Library Prep, Tagmentation | Genomic DNA | Illumina DNA PCR Free, Tag | 25-2000 | 30 | 24 | This kit provides a “low-input” protocol for small input mass. It will also self-normalise samples above 300 ng. |
| Illumina DNA Prep with Enrichment | Genomic DNA | Illumina DNA Prep with Enrichment | 100-500 | 30 | 16? | Also suitable for FFPE extracted material using a modified protocol. |
| ThruPLEX DNA-Seq? | ChIP'ed DNA | Thruplex DNA Seq? | 5-200? | 30 | 24 | |
| Watchmaker RNA with Polaris depletion | Total RNA | Other | 1-1000 | 11? | Any number | Also suitable for FFPE extracted material using a modified protocol. |