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Table of Contents
Submission Form Guidelines for Direct Sequencing on the NextSeq 2000
Direct sequencing is available only to one group CRUK-CI who have bought their own sequencer. If you know nothing about this, then this page is not for you and you should refer to the Sequencing Only submissions help.
Complete the Pool Information Fields
The direct sequencing form is as small as it can be to allow the processing of the run and delivery of correctly processed files. Many of the fields from the SLX submission form are not necessary if you are setting up the sequencer yourself, though the information may need to be supplied on the sequencer itself. Any problems that come up from operating the sequencer incorrectly are, to be blunt, yours!
- Fill out the SLX identifier which is printed on the tube containing your library (SLX-xxxxx).
- Select library type from the drop down menu. Some library types have special handling for processing the data, so to get your data easily please pick the correct one. Important: if submitting for the library types “Amplicon low diversity”, “Amplicon high diversity” or “Bisulphite” please read table 3 of the standard sequencing help regarding PhiX spike-in percentage.
Complete the Sample Information Table
- Complete a separate row with a sample name and index name for each sample within the pool. For example, a pool of six libraries should use six rows.