CRUK-CI Genomics Help

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submission:slx_submissions [2026/03/12 10:55] – [Complete the Pool Information Fields] Johanna Barbierisubmission:slx_submissions [2026/05/15 13:10] (current) – [Further information] Johanna Barbieri
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 ==== Make the Submission ==== ==== Make the Submission ====
  
-  - Run your completed submission form through this submission application. Any errors will be reported back to you for correction. If there are no errors, you will be asked to confirm your submission to the Genomics' LIMS system.+  - Run your completed submission form through this [[https://genomicsequencing.cruk.cam.ac.uk/sequencing/submit/UploadSubmission.action|submission application]]. Any errors will be reported back to you for correction. If there are no errors, you will be asked to confirm your submission to the Genomics' LIMS system.
   - Once your submission is complete, bring your SLX tube(s) to Genomics lab 026 or the Cambridge Institute reception for our external collaborators. Reception can accept tubes between 09:30-16:30 Monday to Friday.   - Once your submission is complete, bring your SLX tube(s) to Genomics lab 026 or the Cambridge Institute reception for our external collaborators. Reception can accept tubes between 09:30-16:30 Monday to Friday.
  
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   - If you must submit a pool with two or more different barcoding kits used in it, please submit with Index Type set to "Unspecified (Other)". The pool can be sequenced to your requirements but it is wholly your responsibility to demultiplex the FASTQ files you will receive. The sequencing service does not support demultiplexing such pools.   - If you must submit a pool with two or more different barcoding kits used in it, please submit with Index Type set to "Unspecified (Other)". The pool can be sequenced to your requirements but it is wholly your responsibility to demultiplex the FASTQ files you will receive. The sequencing service does not support demultiplexing such pools.
   - If you submit a sample for **multiple lanes** of NovaSeq X, **__we strongly suggest running a MiSeq i100__** to help us optimise loading on the instrument. Failing that, __we can't guaranty optimal results__.   - If you submit a sample for **multiple lanes** of NovaSeq X, **__we strongly suggest running a MiSeq i100__** to help us optimise loading on the instrument. Failing that, __we can't guaranty optimal results__.
 +  - Please bear in mind that the size of sequencing data can be very large (especially for multiple lanes of NovaSeq X 25B). Make sure you have enough space set aside to download the data.
  
  
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 |NovaSeq X 25B | 5-10nM |30µl | |NovaSeq X 25B | 5-10nM |30µl |
  
-There is no standard workflow available, like on the NovaSeq 6000, only “Xp”.+Please do not submit molarities above 50nM.
  
 ==== Yields ==== ==== Yields ====