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| submission:slx_submissions [2026/01/08 13:10] – [Yields] Johanna Barbieri | submission:slx_submissions [2026/03/13 14:42] (current) – [Submission Guidelines] Johanna Barbieri | ||
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| - If you are submitting for unspecified indexing where you will demultiplex yourself, you must specify the **index lengths** required for each index. _We cannot accept your library unless we have this information_. | - If you are submitting for unspecified indexing where you will demultiplex yourself, you must specify the **index lengths** required for each index. _We cannot accept your library unless we have this information_. | ||
| - Enter the average library length including insert and adapters (see point 4 of further information). | - Enter the average library length including insert and adapters (see point 4 of further information). | ||
| - | - Select required option for **custom primers**. See the custom primers guide for more information. | + | - Select required option for **custom primers**. See the**[[customprimers| |
| - Select **sample type, sample source and species** from the drop down menus. | - Select **sample type, sample source and species** from the drop down menus. | ||
| - Complete **billing information**. This is critical for correct invoicing: please follow the popup instructions in the field. Only enter a **PO number** if applicable to your department or organisation. **Leave PO number blank if it does not apply!** Resist the temptation to put " | - Complete **billing information**. This is critical for correct invoicing: please follow the popup instructions in the field. Only enter a **PO number** if applicable to your department or organisation. **Leave PO number blank if it does not apply!** Resist the temptation to put " | ||
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| * MiSeq: 15µl of your pooled library at 10-20nM. | * MiSeq: 15µl of your pooled library at 10-20nM. | ||
| + | * NextSeq: 15µl of your pooled library at 10-20nM. | ||
| * MiSeq Express: 15µl at 4nM precisely. | * MiSeq Express: 15µl at 4nM precisely. | ||
| * NovaSeq submissions dependent upon flowcell type: | * NovaSeq submissions dependent upon flowcell type: | ||
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| |NovaSeq X 25B | 5-10nM |30µl | | |NovaSeq X 25B | 5-10nM |30µl | | ||
| - | There is no standard workflow available, like on the NovaSeq 6000, only “Xp”. | + | Please do not submit molarities above 50nM. |
| ==== Yields ==== | ==== Yields ==== | ||