Differences
This shows you the differences between two versions of the page.
| Both sides previous revisionPrevious revisionNext revision | Previous revision | ||
| submission:slx_submissions [2026/01/08 13:10] – [Yields] Johanna Barbieri | submission:slx_submissions [2026/05/15 13:10] (current) – [Further information] Johanna Barbieri | ||
|---|---|---|---|
| Line 20: | Line 20: | ||
| - If you are submitting for unspecified indexing where you will demultiplex yourself, you must specify the **index lengths** required for each index. _We cannot accept your library unless we have this information_. | - If you are submitting for unspecified indexing where you will demultiplex yourself, you must specify the **index lengths** required for each index. _We cannot accept your library unless we have this information_. | ||
| - Enter the average library length including insert and adapters (see point 4 of further information). | - Enter the average library length including insert and adapters (see point 4 of further information). | ||
| - | - Select required option for **custom primers**. See the custom primers guide for more information. | + | - Select required option for **custom primers**. See the**[[customprimers| |
| - Select **sample type, sample source and species** from the drop down menus. | - Select **sample type, sample source and species** from the drop down menus. | ||
| - Complete **billing information**. This is critical for correct invoicing: please follow the popup instructions in the field. Only enter a **PO number** if applicable to your department or organisation. **Leave PO number blank if it does not apply!** Resist the temptation to put " | - Complete **billing information**. This is critical for correct invoicing: please follow the popup instructions in the field. Only enter a **PO number** if applicable to your department or organisation. **Leave PO number blank if it does not apply!** Resist the temptation to put " | ||
| Line 39: | Line 39: | ||
| ==== Make the Submission ==== | ==== Make the Submission ==== | ||
| - | - Run your completed submission form through this submission application. Any errors will be reported back to you for correction. If there are no errors, you will be asked to confirm your submission to the Genomics' | + | - Run your completed submission form through this [[https:// |
| - Once your submission is complete, bring your SLX tube(s) to Genomics lab 026 or the Cambridge Institute reception for our external collaborators. Reception can accept tubes between 09:30-16:30 Monday to Friday. | - Once your submission is complete, bring your SLX tube(s) to Genomics lab 026 or the Cambridge Institute reception for our external collaborators. Reception can accept tubes between 09:30-16:30 Monday to Friday. | ||
| Line 50: | Line 50: | ||
| - If you must submit a pool with two or more different barcoding kits used in it, please submit with Index Type set to " | - If you must submit a pool with two or more different barcoding kits used in it, please submit with Index Type set to " | ||
| - If you submit a sample for **multiple lanes** of NovaSeq X, **__we strongly suggest running a MiSeq i100__** to help us optimise loading on the instrument. Failing that, __we can't guaranty optimal results__. | - If you submit a sample for **multiple lanes** of NovaSeq X, **__we strongly suggest running a MiSeq i100__** to help us optimise loading on the instrument. Failing that, __we can't guaranty optimal results__. | ||
| + | - Please bear in mind that the size of sequencing data can be very large (especially for multiple lanes of NovaSeq X 25B). Make sure you have enough space set aside to download the data. | ||
| Line 55: | Line 56: | ||
| * MiSeq: 15µl of your pooled library at 10-20nM. | * MiSeq: 15µl of your pooled library at 10-20nM. | ||
| + | * NextSeq: 15µl of your pooled library at 10-20nM. | ||
| * MiSeq Express: 15µl at 4nM precisely. | * MiSeq Express: 15µl at 4nM precisely. | ||
| * NovaSeq submissions dependent upon flowcell type: | * NovaSeq submissions dependent upon flowcell type: | ||
| Line 65: | Line 67: | ||
| |NovaSeq X 25B | 5-10nM |30µl | | |NovaSeq X 25B | 5-10nM |30µl | | ||
| - | There is no standard workflow available, like on the NovaSeq 6000, only “Xp”. | + | Please do not submit molarities above 50nM. |
| ==== Yields ==== | ==== Yields ==== | ||