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| submission:slx_submissions [2025/10/07 13:36] – [Further information] Johanna Barbieri | submission:slx_submissions [2026/03/13 14:42] (current) – [Submission Guidelines] Johanna Barbieri | ||
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| - If you are submitting for unspecified indexing where you will demultiplex yourself, you must specify the **index lengths** required for each index. _We cannot accept your library unless we have this information_. | - If you are submitting for unspecified indexing where you will demultiplex yourself, you must specify the **index lengths** required for each index. _We cannot accept your library unless we have this information_. | ||
| - Enter the average library length including insert and adapters (see point 4 of further information). | - Enter the average library length including insert and adapters (see point 4 of further information). | ||
| - | - Select required option for **custom primers**. See the custom primers guide for more information. | + | - Select required option for **custom primers**. See the**[[customprimers| |
| - Select **sample type, sample source and species** from the drop down menus. | - Select **sample type, sample source and species** from the drop down menus. | ||
| - Complete **billing information**. This is critical for correct invoicing: please follow the popup instructions in the field. Only enter a **PO number** if applicable to your department or organisation. **Leave PO number blank if it does not apply!** Resist the temptation to put " | - Complete **billing information**. This is critical for correct invoicing: please follow the popup instructions in the field. Only enter a **PO number** if applicable to your department or organisation. **Leave PO number blank if it does not apply!** Resist the temptation to put " | ||
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| * MiSeq: 15µl of your pooled library at 10-20nM. | * MiSeq: 15µl of your pooled library at 10-20nM. | ||
| + | * NextSeq: 15µl of your pooled library at 10-20nM. | ||
| * MiSeq Express: 15µl at 4nM precisely. | * MiSeq Express: 15µl at 4nM precisely. | ||
| * NovaSeq submissions dependent upon flowcell type: | * NovaSeq submissions dependent upon flowcell type: | ||
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| |NovaSeq X 25B | 5-10nM |30µl | | |NovaSeq X 25B | 5-10nM |30µl | | ||
| - | There is no standard workflow available, like on the NovaSeq 6000, only “Xp”. | + | Please do not submit molarities above 50nM. |
| ==== Yields ==== | ==== Yields ==== | ||
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| | ^MiSeq |^NextSeq 2000 |||^MiSeq i100||^NovaSeq X Plus ^|| | | ^MiSeq |^NextSeq 2000 |||^MiSeq i100||^NovaSeq X Plus ^|| | ||
| - | | |Standard (M) | Nano (M) | P1 (M) | P2 (M) | P3 (B) | P4 (B)| 5M (M) | 25M (M) || 1.5B (M) | 10B (B) | 25B (B)| | + | | |Standard (M) | Nano (M) | P1 (M) | P2 (M) | P3 (B) | P4 (B)| 5M (M) | 25M (M) || 1.5B (B) | 10B (B) | 25B (B)| |
| - | ^Specifications | 15-25 | 1 |100|400|1.2|1.8|5 | 25|| 750 | 1.25 | 3.125 | | + | ^Specifications | 15-25 | 1 |100|400|1.2|1.8|5 | 25|| 0.750 | 1.25 | 3.125 | |
| - | ^ Typical performance | 15-28 | ≤1 | 100 | 400|1.2|1.8| 6 | 25 || 1000| 1.25 | 3.125 | | + | ^ Typical performance | 15-28 | ≤1 | 100 | 400|1.2|1.8| 6 | 25 || 1| 1.4| 3.9 | |
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| - | ^Library type ^MiSeq ^NovaSeq 6000 ^NovaSeq X Plus | | + | ^Library type ^MiSeq ^MiSeq i100 ^NovaSeq X Plus | |
| - | |Bisulphite Converted | 5% | ≥5%* | ≥5%* | | + | |Bisulphite Converted | 5% | 5%* | ≥5%* | |
| - | |Amplicon Low-Diversity | 10% | ≥5%* | 10-20%* | | + | |Amplicon Low-Diversity | 10% | 5%* | 10-20%* | |
| - | |Amplicon High-Diversity | 5% | 1% | 1% | | + | |Amplicon High-Diversity | 5% | 2% | 2% | |
| - | | All other library types | 5% | 1% | 2% | | + | | All other library types | 5% | 2% | 2% | |
| * Optimisation may be required. | * Optimisation may be required. | ||