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| submission:ont_submissions_lps [2025/02/11 15:40] – [Submission guidelines] Johanna Barbieri | submission:ont_submissions_lps [2025/02/11 15:41] (current) – [Adjacent Workflows] Johanna Barbieri | ||
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| |Ligation PCR Barcoding| PCR Barcoding of gDNA/ PCR product allowing for multiplexing on same flow cell| 100-200fmol of each sample or each first round PCR product with tailed primers |PCR product| 45ul| | |Ligation PCR Barcoding| PCR Barcoding of gDNA/ PCR product allowing for multiplexing on same flow cell| 100-200fmol of each sample or each first round PCR product with tailed primers |PCR product| 45ul| | ||
| |Multiplex Ligation Sequencing| PCR-free multiplexing of dsDNA samples such as gDNA and amplicons| 1ug per sample |gDNA | 12ul| | |Multiplex Ligation Sequencing| PCR-free multiplexing of dsDNA samples such as gDNA and amplicons| 1ug per sample |gDNA | 12ul| | ||
| - | |Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) per sample to be barcoded |DNA | in 11.5ul| | + | |Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) per sample to be barcoded |DNA | 11.5ul| |
| |Rapid Barcoding| Rapid Barcoding of gDNA, quick protocol that allows multiplexing of samples on the same flow cell| 200ng per sample |gDNA | up to 10ul| | |Rapid Barcoding| Rapid Barcoding of gDNA, quick protocol that allows multiplexing of samples on the same flow cell| 200ng per sample |gDNA | up to 10ul| | ||
| |Rapid Barcoding Plasmids| Rapid Barcoding of plasmids, quick protocol that allows multiplexing of plasmids on the same flow cell| 50ng per sample |HMW plasmid DNA | 9ul| | |Rapid Barcoding Plasmids| Rapid Barcoding of plasmids, quick protocol that allows multiplexing of plasmids on the same flow cell| 50ng per sample |HMW plasmid DNA | 9ul| | ||