Differences

This shows you the differences between two versions of the page.

--- submission:ont_submissions_lps [2025/02/11 15:23] – [Adjacent Workflows] Johanna Barbieri
+++ submission:ont_submissions_lps [2025/02/11 15:41] (current) – [Adjacent Workflows] Johanna Barbieri
@@ Line 30: / Line 30: @@
 |Ligation PCR Barcoding| PCR Barcoding of gDNA/ PCR product allowing for multiplexing on same flow cell| 100-200fmol of each sample or each first round PCR product with tailed primers |PCR product| 45ul|
 |Multiplex Ligation Sequencing| PCR-free multiplexing of dsDNA samples such as gDNA and amplicons| 1ug per sample |gDNA  | 12ul|
-|Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) per sample to be barcoded |DNA | in 11.5ul|
+|Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) per sample to be barcoded |DNA | 11.5ul|
 |Rapid Barcoding| Rapid Barcoding of gDNA, quick protocol that allows multiplexing of samples on the same flow cell| 200ng per sample |gDNA  | up to 10ul|
 |Rapid Barcoding Plasmids| Rapid Barcoding of plasmids, quick protocol that allows multiplexing of plasmids on the same flow cell| 50ng per sample |HMW plasmid DNA  | 9ul|
@@ Line 39: / Line 39: @@
-===== Submission guidelines =====
+==== Complete the Pool Information Fields ====
+  * Please submit your samples in 1.5mL tubes, clearly and concisely named.
+  * Complete the submission form:
+     * Complete __Billing Information__. This is essential for correct invoicing: please follow the popup instructions in the field. Only enter a PO Number if required by your department or organisation. __Leave PO Number blank if it does not apply__ (resist the temptation to put "N/A" or "none": it will complicate your department's invoice).
+     * The __RIN__ field is only relevant for RNA submissions.
+     * Leave unused sample rows empty.
+  * Run your completed submission form through this submission application. Be sure to click the "Confirm Submission" button to finish the submission process.
+  * Hand your sample to a member of Genomics.