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| Both sides previous revisionPrevious revisionNext revision | Previous revision | ||
| submission:ont_submissions_lps [2025/02/11 11:25] – [Adjacent Workflows] Johanna Barbieri | submission:ont_submissions_lps [2025/02/11 15:41] (current) – [Adjacent Workflows] Johanna Barbieri | ||
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| |Ligation PCR Barcoding| PCR Barcoding of gDNA/ PCR product allowing for multiplexing on same flow cell| 100-200fmol of each sample or each first round PCR product with tailed primers |PCR product| 45ul| | |Ligation PCR Barcoding| PCR Barcoding of gDNA/ PCR product allowing for multiplexing on same flow cell| 100-200fmol of each sample or each first round PCR product with tailed primers |PCR product| 45ul| | ||
| |Multiplex Ligation Sequencing| PCR-free multiplexing of dsDNA samples such as gDNA and amplicons| 1ug per sample |gDNA | 12ul| | |Multiplex Ligation Sequencing| PCR-free multiplexing of dsDNA samples such as gDNA and amplicons| 1ug per sample |gDNA | 12ul| | ||
| - | |Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) per sample to be barcoded |DNA | in 11.5ul| | + | |Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) per sample to be barcoded |DNA | 11.5ul| |
| |Rapid Barcoding| Rapid Barcoding of gDNA, quick protocol that allows multiplexing of samples on the same flow cell| 200ng per sample |gDNA | up to 10ul| | |Rapid Barcoding| Rapid Barcoding of gDNA, quick protocol that allows multiplexing of samples on the same flow cell| 200ng per sample |gDNA | up to 10ul| | ||
| |Rapid Barcoding Plasmids| Rapid Barcoding of plasmids, quick protocol that allows multiplexing of plasmids on the same flow cell| 50ng per sample |HMW plasmid DNA | 9ul| | |Rapid Barcoding Plasmids| Rapid Barcoding of plasmids, quick protocol that allows multiplexing of plasmids on the same flow cell| 50ng per sample |HMW plasmid DNA | 9ul| | ||
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| |Direct RNA Sequence Specific|Kit allows sequence-specific sequencing of native RNA|1ug |total RNA | 8.5ul| | |Direct RNA Sequence Specific|Kit allows sequence-specific sequencing of native RNA|1ug |total RNA | 8.5ul| | ||
| |cDNA-PCR Barcoding|Sequencing of multiple cDNA samples using strand-switching and barcoding for multiplex on the same flow cell|10ng enriched RNA (Poly(A) or ribodepleted per sample \\ or 500ng total RNA per sample | RNA| 10ul| | |cDNA-PCR Barcoding|Sequencing of multiple cDNA samples using strand-switching and barcoding for multiplex on the same flow cell|10ng enriched RNA (Poly(A) or ribodepleted per sample \\ or 500ng total RNA per sample | RNA| 10ul| | ||
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| + | ==== Complete the Pool Information Fields ==== | ||
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| + | * Please submit your samples in 1.5mL tubes, clearly and concisely named. | ||
| + | * Complete the submission form: | ||
| + | * Complete __Billing Information__. This is essential for correct invoicing: please follow the popup instructions in the field. Only enter a PO Number if required by your department or organisation. __Leave PO Number blank if it does not apply__ (resist the temptation to put " | ||
| + | * The __RIN__ field is only relevant for RNA submissions. | ||
| + | * Leave unused sample rows empty. | ||
| + | * Run your completed submission form through this submission application. Be sure to click the " | ||
| + | * Hand your sample to a member of Genomics. | ||
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