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--- submission:ont_submissions_lps [2025/02/10 14:08] – [Main Workflows] Johanna Barbieri
+++ submission:ont_submissions_lps [2025/02/11 15:41] (current) – [Adjacent Workflows] Johanna Barbieri
@@ Line 22: / Line 22: @@
-^ ONT Adjacent Workflows ^Details ^Recommended inputs^
+^ ONT Adjacent Workflows ^Details ^Recommended inputs ^Nucleic acid ^Volume^
-|Ligation Amplicons | Ligation sequencing of amplicons | 100-200 fmol of amplicon DNA in up to 49ul|
+|Ligation Amplicons | Ligation sequencing of amplicons | 100-200 fmol | amplicon DNA | up to 49ul|
-|Ligation Low-input by PCR| Ligation sequencing of low input gDNA using PCR adaptors| 100-200 fmol of gDNA in up to 48ul|
+|Ligation Low-input by PCR| Ligation sequencing of low input gDNA using PCR adaptors| 100-200 fmol | gDNA | up to 48ul|
-|Ligation Direct cDNA| Sequencing of  cDNA samples using RT and strand-switching | 100 ng Poly(A)+ RNA OR                              1 µg of total RNA in 7.5ul|
+|Ligation Direct cDNA| Sequencing of  cDNA samples using RT and strand-switching | 100 ng Poly(A)+ RNA\\ OR\\ 1 µg of total RNA |RNA| 7.5ul|
-|Ligation gDNA Whole Genome Amp| Whole genome amplification (WGA) of genomic DNA using the Ligation Sequencing Kit (SQK-LSK114) and the QIAGEN REPLI-g Midi kit.| 50 pg high molecular weight genomic DNA in up to 4ul|
+|Ligation gDNA Whole Genome Amp| Whole genome amplification (WGA) of genomic DNA using the Ligation Sequencing Kit (SQK-LSK114) and the QIAGEN REPLI-g Midi kit.| 50 pg |high molecular weight genomic DNA | up to 4ul|
-|Ligation 10X Genomics  Single-cell Transcriptomics| Sequencing of full-length transcripts generated by 10X Genomics 3'/5'/Visium FF PolyA capture using custom oligos to enrich.| 10ng cDNA in up to 21ul|
+|Ligation 10X Genomics  Single-cell Transcriptomics| Sequencing of full-length transcripts generated by 10X Genomics 3'/5'/Visium FF PolyA capture using custom oligos to enrich.| 10ng | cDNA | up to 21ul|
-|Ligation PCR Barcoding| PCR Barcoding of gDNA/ PCR product allowing for multiplexing on same flow cell| 100-200fmol of each sample or each first round PCR product with tailed primers in 45ul|
+|Ligation PCR Barcoding| PCR Barcoding of gDNA/ PCR product allowing for multiplexing on same flow cell| 100-200fmol of each sample or each first round PCR product with tailed primers |PCR product| 45ul|
-|Multiplex Ligation Sequencing| PCR-free multiplexing of dsDNA samples such as gDNA and amplicons| 1ug gDNA per sample in 12ul|
+|Multiplex Ligation Sequencing| PCR-free multiplexing of dsDNA samples such as gDNA and amplicons| 1ug per sample |gDNA  | 12ul|
-|Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) DNA per sample to be barcoded in 11.5ul|
+|Native Barcoding Amplicons| Native barcoding of amplicons|200 fmol (130 ng for 1 kb amplicons) per sample to be barcoded |DNA | 11.5ul|
-|Rapid Barcoding| Rapid Barcoding of gDNA, quick protocol that allows multiplexing of samples on the same flow cell| 200ng gDNA per sample in up to 10ul|
+|Rapid Barcoding| Rapid Barcoding of gDNA, quick protocol that allows multiplexing of samples on the same flow cell| 200ng per sample |gDNA  | up to 10ul|
-|Rapid Barcoding Plasmids| Rapid Barcoding of plasmids, quick protocol that allows multiplexing of plasmids on the same flow cell| 50ng HMW plasmid DNA per sample in 9ul|
+|Rapid Barcoding Plasmids| Rapid Barcoding of plasmids, quick protocol that allows multiplexing of plasmids on the same flow cell| 50ng per sample |HMW plasmid DNA  | 9ul|
-|Rapid Barcoding Amplicons| Rapid Barcoding of amplicons, quick protocol that allows multiplexing of amplicons on the same flow cell|50ng amplicon DNA per sample (500bp - 5kb amplicon size) in 9ul|
+|Rapid Barcoding Amplicons| Rapid Barcoding of amplicons, quick protocol that allows multiplexing of amplicons on the same flow cell|50ng per sample (500bp - 5kb amplicon size) |amplicon DNA  | 9ul|
-|Direct RNA Sequence Specific|Kit allows sequence-specific sequencing of native RNA|1ug total RNA in 8.5ul|
+|Direct RNA Sequence Specific|Kit allows sequence-specific sequencing of native RNA|1ug |total RNA | 8.5ul|
-|cDNA-PCR Barcoding|Sequencing of multiple cDNA samples using strand-switching and barcoding for multiplex on the same flow cell|10ng enriched RNA (Poly(A) or ribodepleted per sample \\or 500ng total RNA per sample in 10ul|
+|cDNA-PCR Barcoding|Sequencing of multiple cDNA samples using strand-switching and barcoding for multiplex on the same flow cell|10ng enriched RNA (Poly(A) or ribodepleted per sample \\ or 500ng total RNA per sample | RNA| 10ul|
+==== Complete the Pool Information Fields ====
+  * Please submit your samples in 1.5mL tubes, clearly and concisely named.
+  * Complete the submission form:
+     * Complete __Billing Information__. This is essential for correct invoicing: please follow the popup instructions in the field. Only enter a PO Number if required by your department or organisation. __Leave PO Number blank if it does not apply__ (resist the temptation to put "N/A" or "none": it will complicate your department's invoice).
+     * The __RIN__ field is only relevant for RNA submissions.
+     * Leave unused sample rows empty.
+  * Run your completed submission form through this submission application. Be sure to click the "Confirm Submission" button to finish the submission process.
+  * Hand your sample to a member of Genomics.