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--- submission:ont_submissions [2024/11/21 16:15] – [Complete the Pool Information Fields] Johanna Barbieri
+++ submission:ont_submissions [2025/04/04 09:16] (current) – Johanna Barbieri
@@ Line 1: / Line 1: @@
-====== Genomics Core Library Preparation Submission ======
+====== Genomics Core ONT Sequencing Submission ======
+The loading requirements for ONT sequencing can be found at the end of each library preparation protocol.
+We ask that you submit at least those requirements (keeping in mind that the more we load the better the runs will be).
-^Library prep kit ^ Submit ^ Submission workflow ^ Quantity range (ng) ^ Volume required per prep (uL)* ^ Sample batch size** ^Notes^
+If your libraries don't meet the ONT loading requirements, please contact us here [[:genomics-helpdesk@cruk.cam.ac.uk|Genomics Helpdesk]].
-|Illumina Stranded mRNA|Total RNA|Illumina Stranded mRNA|25-1000|25|Any number|This kit supports various input mass; normalise where possible.|
-|NEBNext Single cell / low input RNA Library Prep Kit for Illumina|Total RNA/cells|NEB low input RNA|2pg - 200ng, or cell(s)|8|24|Please discuss your input requirements prior to submission.|
+You'll find ONT library preparation protocols [[https://nanoporetech.com/documentation/results?category=prepare&topic=library-prep-protocols|here]] and our own Library preparation guidelines [[submission:ont_submissions_lps|here]].
-|Illumina DNA PCR-Free Library Prep, Tagmentation|Genomic DNA|Illumina DNA PCR Free, Tag|25-2000|30|24|This kit provides a "low-input" protocol for small input mass. It will also self-normalise samples above 300 ng.|
-|Illumina DNA Prep with Enrichment|Genomic DNA|Illumina DNA Prep with Enrichment|100-500|30|16?|Also suitable for FFPE extracted material using a modified protocol.|
-|ThruPLEX DNA-Seq?|ChIP'ed DNA|Thruplex DNA Seq?|5-200?|30|24|
-|Watchmaker RNA with Polaris depletion| Total RNA| Other| 1-1000| 11?| Any number|Also suitable for FFPE extracted material using a modified protocol.|\\
-Please provide an additional 15% volume per well.\\
-We run a positive and negative control with every library prep.
-==== Complete the Pool Information Fields ====
-- 96 well barcoded plates and seals are available from the Genomics Core (Room 026). Barcoded plates can also be obtained either from CRUK reception or via UMS for University users.