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| submission:ont_submissions [2024/11/21 16:08] – Johanna Barbieri | submission:ont_submissions [2025/04/04 09:16] (current) – Johanna Barbieri | ||
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| - | ====== Genomics Core Library Preparation | + | ====== Genomics Core ONT Sequencing |
| + | The loading requirements for ONT sequencing can be found at the end of each library preparation protocol. | ||
| + | We ask that you submit at least those requirements (keeping in mind that the more we load the better the runs will be). | ||
| - | ^Library prep kit ^ Submit ^ Submission workflow ^ Quantity range (ng) ^ Volume required per prep (uL)* ^ Sample batch size** ^Notes^ | + | If your libraries don't meet the ONT loading |
| - | |Illumina Stranded mRNA|Total RNA|Illumina Stranded mRNA|25-1000|25|Any number|This kit supports various input mass; normalise where possible.| | + | |
| - | |NEBNext Single cell / low input RNA Library Prep Kit for Illumina|Total RNA/ | + | You'll find ONT library preparation protocols [[https:// |
| - | |Illumina DNA PCR-Free Library Prep, Tagmentation|Genomic DNA|Illumina DNA PCR Free, Tag|25-2000|30|24|This kit provides a " | + | |
| - | |Illumina DNA Prep with Enrichment|Genomic DNA|Illumina DNA Prep with Enrichment|100-500|30|16? | + | |
| - | |ThruPLEX DNA-Seq? | + | |
| - | |Watchmaker RNA with Polaris depletion| Total RNA| Other| 1-1000| 11?| Any number|Also suitable for FFPE extracted material using a modified protocol.| | + | |
| - | * Please provide an additional 15% volume per well. | + | |
| - | ** We run a positive and negative control with every library prep. | + | |