CRUK-CI Genomics Help

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revision		Previous revision
submission:lps_submissions [2025/02/11 16:29] – [Complete the Pool Information Fields] Johanna Barbierisubmission:lps_submissions [2026/02/18 11:23] (current) – [Genomics Core Library Preparation Submission] Johanna Barbieri
Line 6: Line 6:
 |NEBNext Single cell / low input RNA Library Prep Kit for Illumina|Total RNA/cells|NEB low input RNA|2pg - 200ng, or cell(s)|8|24|Please discuss your input requirements prior to submission.| |NEBNext Single cell / low input RNA Library Prep Kit for Illumina|Total RNA/cells|NEB low input RNA|2pg - 200ng, or cell(s)|8|24|Please discuss your input requirements prior to submission.|
 |Illumina DNA PCR-Free Library Prep, Tagmentation|Genomic DNA|Illumina DNA PCR Free, Tag|25-2000|30|24|This kit provides a "low-input" protocol for small input mass. It will also self-normalise samples above 300 ng.| |Illumina DNA PCR-Free Library Prep, Tagmentation|Genomic DNA|Illumina DNA PCR Free, Tag|25-2000|30|24|This kit provides a "low-input" protocol for small input mass. It will also self-normalise samples above 300 ng.|
-|Illumina DNA Prep with Enrichment|Genomic DNA|Illumina DNA Prep with Enrichment|100-500|30|16?|Also suitable for FFPE extracted material using a modified protocol.|+|Illumina DNA Prep with Enrichment|Genomic DNA|Illumina DNA Prep with Enrichment|10-1000|30|16|Also suitable for FFPE extracted material using a modified protocol.|
 |ThruPLEX DNA-Seq|ChIP'ed DNA|Thruplex DNA Seq|50pg - 50ng |10|24| |ThruPLEX DNA-Seq|ChIP'ed DNA|Thruplex DNA Seq|50pg - 50ng |10|24|
 |Watchmaker RNA with Polaris depletion| Total RNA| Other| 1-1000| 18| Any number|Also suitable for FFPE extracted material using a modified protocol.| |Watchmaker RNA with Polaris depletion| Total RNA| Other| 1-1000| 18| Any number|Also suitable for FFPE extracted material using a modified protocol.|
  
-Please provide an additional 15% volume per well.\\+*Please provide an additional 15% volume per well.\\
 We run a positive and negative control with every library prep. We run a positive and negative control with every library prep.
  
Line 41: Line 41:
   - We do not specify a minimum quantity for a ChIP-seq experiment; please submit what you have. Always include at least one input control.   - We do not specify a minimum quantity for a ChIP-seq experiment; please submit what you have. Always include at least one input control.
   - Please upload QC data to the relevant project in our LIMS.   - Please upload QC data to the relevant project in our LIMS.
 +
 +
 +==== Library Prep Submission Recommendations ====
 +
 +  - This is the[[https://genomicshelp.cruk.cam.ac.uk/forms/CRUKCI_LPS_Submission.xlsx|submission form for library prep]] specifically. Do not use this form to submit sequencing ready samples.
 +  - There are different batch sizes depending on library prep, please see our price card or the table above for details.
 +  - Researchers from within the CRUK-CI are strongly encouraged to attend an experimental design meeting prior to collecting samples for library prep submission.
 +  - Contact[[:CRIExperimentalDesign@cruk.cam.ac.uk|CRIExperimentalDesign@cruk.cam.ac.uk]] to book a free experimental design session.
 +  - If you have any questions, contact the[[:genomics-helpdesk@cruk.cam.ac.uk|Genomics Helpdesk]].