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| submission:customprimers [2026/03/12 10:57] – [Instructions for Custom Primers] Johanna Barbieri | submission:customprimers [2026/10/02 14:47] (current) – [Instructions for Custom Primers] Johanna Barbieri | ||
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| - Label the side of each tube with the same information, | - Label the side of each tube with the same information, | ||
| - Provide an aliquot containing: | - Provide an aliquot containing: | ||
| - | - 7µl volume per lane of MiSeq submitted. | + | - 10µl volume per lane of MiSeq i100 submitted. |
| - 35µl volume for each flowcell of NovaSeq X 1.5B submitted. | - 35µl volume for each flowcell of NovaSeq X 1.5B submitted. | ||
| - 55µl volume for each flowcell of NovaSeq X 10B or 25B submitted. | - 55µl volume for each flowcell of NovaSeq X 10B or 25B submitted. | ||
| + | - 10µl volume for each NextSeq flowcell. | ||
| - When you submit, enter the following in the Submission Comments field:// | - When you submit, enter the following in the Submission Comments field:// | ||
| - You will also need to select in your submission form if these primers are to be **spiked in**to the Illumina primers or if they are to be used **instead of** the Illumina primers. Please note that if you require PhIX to be sequenced, then the Illumina primers must also be used. In this example, you would select for the primers to be spiked in. This would be important for library types of low diversity. | - You will also need to select in your submission form if these primers are to be **spiked in**to the Illumina primers or if they are to be used **instead of** the Illumina primers. Please note that if you require PhIX to be sequenced, then the Illumina primers must also be used. In this example, you would select for the primers to be spiked in. This would be important for library types of low diversity. | ||