CRUK-CI Genomics Help

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submission:customprimers [2024/10/21 15:19] – created Johanna Barbierisubmission:customprimers [2026/03/12 10:58] (current) – [Instructions for Custom Primers] Johanna Barbieri
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   - Label the side of each tube with the same information, plus the concentration (50µM).   - Label the side of each tube with the same information, plus the concentration (50µM).
   - Provide an aliquot containing:   - Provide an aliquot containing:
-    - 7µl volume per lane of MiSeq submitted. +    - 10µl volume per lane of MiSeq i100 submitted. 
-    - 35µl volume for each flowcell of NovaSeq SP, S1 or S2 submitted. +    - 35µl volume for each flowcell of NovaSeq X 1.5B submitted. 
-    - 50µl volume for each flowcell of NovaSeq S4 submitted.+    - 55µl volume for each flowcell of NovaSeq X 10B or 25B submitted.
   - When you submit, enter the following in the Submission Comments field://Requires custom primer: "MetR1" for read 1, and "MetIN" for Index.//   - When you submit, enter the following in the Submission Comments field://Requires custom primer: "MetR1" for read 1, and "MetIN" for Index.//
-  - You will also need to select in your submission form if these primers are to be spiked into the Illumina primers or if they are to be used instead of the Illumina primers. Please note that if you require PhIX to be sequenced, then the Illumina primers must also be used. In this example, you would select for the primers to be spiked in. This would be important for library types of low diversity.+  - You will also need to select in your submission form if these primers are to be **spiked in**to the Illumina primers or if they are to be used **instead of** the Illumina primers. Please note that if you require PhIX to be sequenced, then the Illumina primers must also be used. In this example, you would select for the primers to be spiked in. This would be important for library types of low diversity.
   - Provide all necessary primer aliquots whenever you submit libraries of this type.   - Provide all necessary primer aliquots whenever you submit libraries of this type.