CRUK-CI Genomics Help

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submission:10x_submissions [2026/04/10 12:50] – [Cell Suspension Advice] Johanna Barbierisubmission:10x_submissions [2026/07/06 13:13] (current) – [Single Cell Submission] Johanna Barbieri
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 In the first instance, contact the single cell team to discuss details of the cells or nuclei submission to ensure they are aware of the experimental conditions and timelines.\\ In the first instance, contact the single cell team to discuss details of the cells or nuclei submission to ensure they are aware of the experimental conditions and timelines.\\
-[[singlecell@cruk.cam.ac.uk|Single cell team]]+[[singlecell@cruk.cam.ac.uk|Single cell team]]\\ 
 +__**It is recommended for any single cell 10x Genomics project to consider a pilot experiment prior to a multi-sample project**__
  
 For 10x Genomics, We ask you to bring your cell suspension or isolated nuclei in the following required concentration in 1x PBS +0.04% BSA. Alternatively, there are other buffers acceptable that 10x Genomics list on their website found here: https://kb.10xgenomics.com/hc/en-us/articles/115001937123-What-buffers-can-be-used-for-washing-and-cell-resuspension. \\ For 10x Genomics, We ask you to bring your cell suspension or isolated nuclei in the following required concentration in 1x PBS +0.04% BSA. Alternatively, there are other buffers acceptable that 10x Genomics list on their website found here: https://kb.10xgenomics.com/hc/en-us/articles/115001937123-What-buffers-can-be-used-for-washing-and-cell-resuspension. \\
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 ^Workflow ^Kit used ^ Sample type ^ Volume loaded (µl) ^Max Target Concentration (cells/µl) ^Volume for User to Submit (µl) ^ # Cells Loaded ^ # Cells Recovered ^ Preferred Eppendorf ^ ^Workflow ^Kit used ^ Sample type ^ Volume loaded (µl) ^Max Target Concentration (cells/µl) ^Volume for User to Submit (µl) ^ # Cells Loaded ^ # Cells Recovered ^ Preferred Eppendorf ^
-|10x Single Cell 3GEX|Next GEM 3'v3.1|Cells/Nuclei| 43.2|370|55|16,000|10,000|1.5ml tubes|+|10x Single Cell 3GEX **(OBSOLETE)**|Next GEM 3'v3.1|Cells/Nuclei| 43.2|370|55|16,000|10,000|1.5ml tubes|
 |10x Single Cell 3GEMX|GEM-X 3'v4|Cells/Nuclei|36.6|683|45|25,000 |20,000  |1.5ml tubes| |10x Single Cell 3GEMX|GEM-X 3'v4|Cells/Nuclei|36.6|683|45|25,000 |20,000  |1.5ml tubes|
-|10x Single Cell 5GEX|Next GEM 5'v2|Cells/Nuclei|39.1|409|45|16,000|10,000|1.5ml tubes|+|10x Single Cell 5GEX **(OBSOLETE)**|Next GEM 5'v2|Cells/Nuclei|39.1|409|45|16,000|10,000|1.5ml tubes|
 |10x Single Cell 5GEMX|GEM-X 5'v3|Cells/Nuclei|37.5|667|45|25,000|20,000|1.5ml tubes| |10x Single Cell 5GEMX|GEM-X 5'v3|Cells/Nuclei|37.5|667|45|25,000|20,000|1.5ml tubes|
 |10x Single Cell 3GEMX OCM 4-plex|GEM-X 3' OCM|Cells/Nuclei|9.6|860|15|8,250|5,000|1.5ml tubes| |10x Single Cell 3GEMX OCM 4-plex|GEM-X 3' OCM|Cells/Nuclei|9.6|860|15|8,250|5,000|1.5ml tubes|