Differences

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--- submission:10x_submissions [2025/02/14 11:41] – [Single Cell Submission] Ania Piskorz
+++ submission:10x_submissions [2026/04/10 12:57] (current) – [Single Cell Submission] Johanna Barbieri
@@ Line 5: / Line 5: @@
 In the first instance, contact the single cell team to discuss details of the cells or nuclei submission to ensure they are aware of the experimental conditions and timelines.\\
-[[singlecell@cruk.cam.ac.uk|Single cell team]]
+[[singlecell@cruk.cam.ac.uk|Single cell team]]\\
+__**It is recommended for any single cell 10x Genomics project to consider a pilot experiment prior to a multi-sample project**__
 For 10x Genomics, We ask you to bring your cell suspension or isolated nuclei in the following required concentration in 1x PBS +0.04% BSA. Alternatively, there are other buffers acceptable that 10x Genomics list on their website found here: https://kb.10xgenomics.com/hc/en-us/articles/115001937123-What-buffers-can-be-used-for-washing-and-cell-resuspension. \\
@@ Line 42: / Line 43: @@
 Samples should be submitted in a clearly labelled tube (see table above) and brought on ice.
-It is recommended for any single cell 10x Genomics project to consider a pilot experiment prior to a multi-sample project. This may not be possible for Parse given the wholistic use of the kit but potentially utilising their Mini kit first may alleviate significant investment waste.
+__**It is recommended for any single cell 10x Genomics project to consider a pilot experiment prior to a multi-sample project**__. This may not be possible for Parse given the wholistic use of the kit but potentially utilising their Mini kit first may alleviate significant investment waste.
 For any other questions you might have about cell preparation, counting and sequencing please see our [[https://genomicsequencing.cruk.cam.ac.uk/sequencing/help/Help!tenxFAQ.action|FAQ]] page.