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| Both sides previous revisionPrevious revisionNext revision | Previous revision | ||
| submission:10x_submissions [2025/02/14 11:41] – [Single Cell Submission] Ania Piskorz | submission:10x_submissions [2026/07/06 13:13] (current) – [Single Cell Submission] Johanna Barbieri | ||
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| In the first instance, contact the single cell team to discuss details of the cells or nuclei submission to ensure they are aware of the experimental conditions and timelines.\\ | In the first instance, contact the single cell team to discuss details of the cells or nuclei submission to ensure they are aware of the experimental conditions and timelines.\\ | ||
| - | [[singlecell@cruk.cam.ac.uk|Single cell team]] | + | [[singlecell@cruk.cam.ac.uk|Single cell team]]\\ |
| + | __**It is recommended for any single cell 10x Genomics project to consider a pilot experiment prior to a multi-sample project**__ | ||
| For 10x Genomics, We ask you to bring your cell suspension or isolated nuclei in the following required concentration in 1x PBS +0.04% BSA. Alternatively, | For 10x Genomics, We ask you to bring your cell suspension or isolated nuclei in the following required concentration in 1x PBS +0.04% BSA. Alternatively, | ||
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| ^Workflow ^Kit used ^ Sample type ^ Volume loaded (µl) ^Max Target Concentration (cells/µl) ^Volume for User to Submit (µl) ^ # Cells Loaded ^ # Cells Recovered ^ Preferred Eppendorf ^ | ^Workflow ^Kit used ^ Sample type ^ Volume loaded (µl) ^Max Target Concentration (cells/µl) ^Volume for User to Submit (µl) ^ # Cells Loaded ^ # Cells Recovered ^ Preferred Eppendorf ^ | ||
| - | |10x Single Cell 3GEX|Next GEM 3' | + | |10x Single Cell 3GEX **(OBSOLETE)**|Next GEM 3' |
| |10x Single Cell 3GEMX|GEM-X 3' | |10x Single Cell 3GEMX|GEM-X 3' | ||
| - | |10x Single Cell 5GEX|Next GEM 5' | + | |10x Single Cell 5GEX **(OBSOLETE)**|Next GEM 5' |
| |10x Single Cell 5GEMX|GEM-X 5' | |10x Single Cell 5GEMX|GEM-X 5' | ||
| |10x Single Cell 3GEMX OCM 4-plex|GEM-X 3' OCM|Cells/ | |10x Single Cell 3GEMX OCM 4-plex|GEM-X 3' OCM|Cells/ | ||
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| Samples should be submitted in a clearly labelled tube (see table above) and brought on ice. | Samples should be submitted in a clearly labelled tube (see table above) and brought on ice. | ||
| - | It is recommended for any single cell 10x Genomics project to consider a pilot experiment prior to a multi-sample project. This may not be possible for Parse given the wholistic use of the kit but potentially utilising their Mini kit first may alleviate significant investment waste. | + | __**It is recommended for any single cell 10x Genomics project to consider a pilot experiment prior to a multi-sample project**__. This may not be possible for Parse given the wholistic use of the kit but potentially utilising their Mini kit first may alleviate significant investment waste. |
| For any other questions you might have about cell preparation, | For any other questions you might have about cell preparation, | ||