CRUK-CI Genomics Help

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submission:10x_submissions [2025/02/14 11:41] – Ania Piskorzsubmission:10x_submissions [2025/02/14 11:41] (current) – [Single Cell Submission] Ania Piskorz
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 ^Workflow ^Kit used ^ Sample type ^Max Number of Cells to Submit ^Max  # Cells Loaded ^ # Cells Recovered ^ Preferred Eppendorf ^ ^Workflow ^Kit used ^ Sample type ^Max Number of Cells to Submit ^Max  # Cells Loaded ^ # Cells Recovered ^ Preferred Eppendorf ^
-|10x Single Cell GEMX Flex Singleplex 1plex|GEMX Flex Singleplex|Cells 1plex/Nuclei|300,000|29,250 |20,000 |1.5ml tubes| +|10x Single Cell GEMX Flex Singleplex 1plex 4 samples|GEMX Flex Singleplex|Cells 1plex/Nuclei|300,000|29,250 |20,000 |1.5ml tubes| 
-|10x Single Cell GEMX Flex Multiplex 4plex|GEMX Flex Multiplex|Cells 4plex/Nuclei|300,000|116,000|80,000 |1.5ml tubes|+|10x Single Cell GEMX Flex Multiplex 4plex 16 samples|GEMX Flex Multiplex|Cells 4plex/Nuclei|300,000|116,000|80,000 |1.5ml tubes|
      
 For Parse, we ask you to utilise an excel sheet that Parse provide to determine both concentration and volume to target for your submissions to us, this includes a dilution step upon our receipt before loading to the first barcoding plate. These excel sheets can be found on Parse's website under the kit you used (Mini/Midi/Mega) as they are different for each. Additionally, we would highly recommend to aliquot part of your samples to count the day before submission without freeze-thawing and allowing you to drop samples off earlier on the day.  For Parse, we ask you to utilise an excel sheet that Parse provide to determine both concentration and volume to target for your submissions to us, this includes a dilution step upon our receipt before loading to the first barcoding plate. These excel sheets can be found on Parse's website under the kit you used (Mini/Midi/Mega) as they are different for each. Additionally, we would highly recommend to aliquot part of your samples to count the day before submission without freeze-thawing and allowing you to drop samples off earlier on the day.